First Report of <i>Mucor irregularis</i> Causing Postharvest Fruit Rot on <i>Garcinia mangostana</i> in China

HomePlant DiseaseAhead of PrintFirst Report Mucor irregularis Causing Postharvest Fruit Rot on Garcinia mangostana in China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst ChinaT. Wang, C. X. Sun, and P. K. ZhuT. Wang†Corresponding author: T. Wang; E-mail Address: [email protected]://orcid.org/0000-0002-1443-9933Nanyang Normal University, Nanyang, Henan Province, 473061, P.R. ChinaSchool Life Sciences, East Shanghai, 200241, ChinaSearch for more papers by this author, SunSchool ChinaXuzhou Academy Agricultural Xuzhou, Jiangsu 221000, Zhuhttps://orcid.org/0000-0003-0635-7839School author AffiliationsAuthors Affiliations Wang1 2 † Sun2 3 Zhu2 1Nanyang 2School 3Xuzhou Published Online:17 Jan 2022https://doi.org/10.1094/PDIS-07-21-1431-PDNAboutSectionsView articlePDFPDF PlusSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleMangosteen (Garcinia L.) is an evergreen fruit tree grown Southeast Asia. The Mangosteen spherical dark purple with a diameter 5 8 cm, hard rind, white flesh. seeds pericarps the have long been utilized as traditional medicine Asia (Ovalle-Magallanes et al. 2017). Surveys were conducted from March April 2019 markets Wujing Town, Shanghai (31°02′33″ N, 121°28′04″ E), one noteworthy postharvest disease was observed. Overall, 116 samples collected five different supermarkets; 10.3% (12) fruits found infected same symptoms during cold storage. subjected pathogen isolation characterization. junction areas between diseased healthy tissues cut into small pieces mm diameter, which surface sterilized 0.5% NaClO solution min, followed rinsing sterile water three times, then placed potato dextrose agar (PDA) plates supplemented 50 μg/ml streptomycin. colony edges resulting fungal cultures moved new PDA purification after days incubation at 28°C. After rounds subculture, total 19 purified isolates obtained, showed similar phenotypes morphological characters. colonies fast growing (25 per day 28°C plates) circular massive aerial hyphae, ochraceous color. Nonseptate hyphae hyaline septa only branching points. Sporangiophores arose or stolons. Sporangiospores hyaline, smooth-walled, mostly subspherical ellipsoidal, measured 1.34 2.73 µm (width) 3.12 6.19 (length) (1.98 ± 0.36 × 4.46 0.69, n = 100) (Álvarez 2011). Three independent (GM-07, GM-16, GM-19) selected further genetic analysis pathogenicity tests. internal transcribed spacer (ITS) D1/D2 region nuclear large subunit ribosomal RNA gene (LSU) amplified primers ITS1/ITS4 NL1/LR3, respectively (Walther 2013). BLASTn analyses ITS (MN081612, MN081613, MN087659) LSU (MN080423, MN080422, MN080432) sequences exhibited 99 100% identity strains HZ640, extr25, CBS977.68 (accession nos. KC461926 MH510278 JX976214 LSU). These features molecular identification indicated that possessed identical characteristics M. Stchigel, Cano, Guarro & Ed. Alvarez To conduct test (Al-Sadi 2014), 20 µl spore suspension (106 spores/ml) inoculated mangosteen syringes, while another injected controls. All kept sealed plastic boxes 12/12 h light/dark illumination cycle 90% relative humidity. about 7 days, materials those original fruits, control remained symptomless. successfully reisolated symptomatic fulfilling Koch’s postulates. Only plant species can be reported so far, including key lime Oman corn (Peng 2015), sorghum Brazil (Lima 2018). our knowledge, first report causing rot China.The author(s) declare no conflict interest.References:Al-Sadi, A. M., 2014. Int. J. Agric. Biol. 16:371. ISI, Google ScholarÁlvarez, E., 2011. Med. Mycol. 49:62. https://doi.org/10.3109/13693786.2010.499521 Crossref, ScholarLima, D. X., 2018. Mycotaxon 133:429. https://doi.org/10.5248/133.429 ScholarOvalle-Magallanes, B., 2017. Food Chem. Toxicol. 109:102. https://doi.org/10.1016/j.fct.2017.08.021 ScholarPeng, D., 2015. Plant Dis. 99:159. https://doi.org/10.1094/PDIS-08-14-0814-PDN Link, ScholarWalther, G., 2013. Persoonia 30:11. https://doi.org/10.3767/003158513X665070 ScholarFunding: This research funded Research Center Provincial Biomass Resource Engineering Technology.The interest.DetailsFiguresLiterature CitedRelated Just PublishedSubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Downloaded 161 times Article History Published: 17 2022First Look: Sep 2021Accepted: 30 Aug 2021 Information© 2022 American Phytopathological SocietyFundingResearch TechnologyKeywordsfungifruittree fruitspathogen detectionThe interest.